Microbial Cell Production

This facility has the mission to provide technical support to research groups in bacteria/yeast cultivation and associated molecular biology techniques. These services are available to all researchers and are run by qualified and experienced technicians.

 

  • Growth of bacteria or yeast on a laboratory-scale in liquid and solid media
  • Growth of bacteria at a bioreactor-scale between 30-300 L
  • Cell disruption up to a volume of 120 mL
  • Assistance in culture media development and optimization
  • Plasmid extraction from Escherichia coli at small or large-scale (commercial kits)
  • Preparation of chemical or electro-competent cells of E. coli, Staphylococcus aureus, and other strains
  • Maintenance and organization of biological collections and primers
  • Cryopreservation of bacterial strains at -80°C
  • Preparation of SDS-PAGE gels
  • Western-Blots and Immunoblotting

The Microbial Cell Production facility serves ITQB NOVA researchers – through ticket requests. Please see the contacts below to book the training on the different equipment, and for other requests. All costs are supported by the research groups.

João Carita – carita@itqb.unl.pt 

Teresa Batista da Silva – teresas@itqb.unl.pt

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