Protein Purification & Characterization
This facility provides assistance and expertise in protein purification using fast pressure liquid chromatography systems and biochemical characterization of proteins. These services are available to all researchers and are run by qualified and experienced technicians. Users can also be trained in order to use AKTA systems independently.
- Bacteria or yeast cell disruption (French-press, sonication or glass beads)
- Crude cell extracts preparation
- Purification using ion exchange (IEX), hydrophobic (HIC), affinity (IMAC) and size exclusion chromatography
- Analysis of protein purity and molecular mass estimation (SDS-PAGE, Blue-native, Tricine or Native electrophoresis)
Western-Blots - Protein content determination (Lowry, BCA, Bradford methods)
- Enzymatic assays
- Characterization of enzymes cofactors using UV-vis spectroscopy
- N-terminal protein and peptide sequencing analysis (Edman degradation method)
- Anaerobic chambers
- UV-vis spectrophotometers
- AKTA Prime Plus
- AKTA Purifier
- AKTA Purifier 10
- AKTA Purifier UCP
The Protein Purification & Characterization facility serves ITQB NOVA researchers – through ticket requests – and is run by qualified and experienced technicians. All costs are supported by the research groups.
Users can also be trained in order to use AKTA systems independently.
Cristina Timóteo – cristina.timoteo@itqb.unl.pt
Teresa Baptista – teresas@itqb.unl.pt
Paula Chicau – chicau@itqb.unl.pt
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